Abstract
Complete autotetraploid plants in three monoembryonic diploid citrus cultivars ('Clementine', 'Hassaku' and 'Hyuganatsu') were produced successfully from small young axillary buds isolated and immersed in 0.1% colchicine solution for 2-6 hours by using the micrografting technique. The induced tetraploidy was associated with the presence of broader and thicker leaves, larger stomata and flowers. Moreover, thorns were produced on the elongating shoots of all the induced tetraploid plants. The signiflcance of this thorniness for the detection of tetraploidy in citrus after colchicine treatment is discussed.
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