PRODUCTION OF BIOGAS FROM CELLULOSE AND BENZOATE USING ANAEROBIC BACTERIAL CONSORTIA

Manisha D R,Karunakar Rao Kudle,M.P.Pratap Rudra,Ramchander Merugu,Singh Singh

doi:10.29121/ijetmr.v4.i12.2017.593

Abstract

Among the energy sources, the depletion of non-renewable energy is of great concern in thepresent days. The non- renewable energy deposits are continuously depleted by humanwithdrawals. An alarming situation of continuous depletion in the natural resources by anincreased consumption of energy, the urge to look out for alternate ways for energyproduction in on the high stand. Anaerobic bacteria are known to be efficient energyproducers. In the present study, the anaerobic bacterial hydrogen production from differentconsortia isolated from different sources was studied. Anaerobic consortia were isolated fromdifferent sources by inoculating into specific media, Beibl and Pfennigs medium anaerobicallyin the light (2000 lux). Bergey’s Manual of Systematic Bacteriology (1994) was adopted foridentification of phototrophic bacteria. Growth was determined using UV-Visspectrophotometer at 660nm. The effect of two major substrates i.e. Benzoate and Cellulosehas been investigated. The amount of biogas produced varied with benzoate and cellulose assubstrates. Among the 10(ten) consortia, for most of the consortia cellulose was confirmed tobe utilized as a major substrate for the production of biogas.

Highlights

Contemporary human society is based on non-renewable fossil fuels, which represents more than 80% of our energy consumption
The anaerobic bacteria hydrogen production from different consortia isolated from ten different sources was studied
Ten day old cultures of photosynthetic bacteria isolated from ten different sources were inoculated (1% v/v) into basal medium containing two different carbon sources i.e. Benzoate and Cellulose with ammonium chloride (0.5%) was used as nitrogen source and B12 as the growth factor

Summary

Introduction

Contemporary human society is based on non-renewable fossil fuels (oil, coal, natural gas), which represents more than 80% of our energy consumption. Worldwide attention is paid to the replacement of the current energy carriers with alternative, renewable ones with rapid technology development. The main component of the biogas is methane, depending on the fermentation substrate it makes up to 50-75% of the gas, in addition it contains carbon dioxide (28-48%) and other gases (~1-2%) for example hydrogen sulphide and nitrogen compounds.

Results

Conclusion