Production of Bioethanol From Kappaphycus alvarezii Algae by Using Pichia kudriavzevii Yeast

Nur Fadillah,W L Taufan ,Muh Syahdan Aska

doi:10.20956/ijab.v2i1.4391

Nur Fadillah, W L Taufan + Show 1 more

Open Access

https://doi.org/10.20956/ijab.v2i1.4391

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Abstract

Kappaphycus alvarezii is a red algae that can be used as alternative raw material for bioethanol production. This is because K. alvarezii contains a high carbohydrate that reaches 60%. This study aims to determine the effect of fermentation nutrition and fermentation duration of hydrolysis results of K. alvarezii algae on pH changes, sugar levels, cell biomass and ethanol content. There are two methods was used in this research, namely hydrolysis method and fermentation method. The hydrolysis method was used α-amylases enzyme which is 150 KNU/L with 0.5%, 1.0%, 1.5% and 2.0% concentrations. The next method, K. alvarezii algae was fermented by Pichia kudriavzevii and used Gandasil-D® as an anorganic suplementation and yeast extract which is 0 g/L, 1 g/L, 2 g/L and 3 g/L. The results showed that K. alvarezii can be fermented into bioethanol after enzymatic hydrolysis process. The optimal ethanol content was produced at 48 hours of incubation duration.

Highlights

K. alvarezii is a leading commodity produced in various countries, one of that is Indonesia whose production reaches 12-18 tons of dry algae/ha/ year (Hayashi et al, 2011; Lee et al, 2016; Ra et al, 2013)
Sugar Levels in Hydrolysis Process The results of the measurement of sugar content showed that algae concentration of 0.5% resulted in average sugar content of 0.56 g/g during 24 and 48 hours incubation
72 hour incubation showed the average of different sugar content with other incubation duration, that is 0,47 g/g

Summary

Introduction

K. alvarezii is a leading commodity produced in various countries, one of that is Indonesia whose production reaches 12-18 tons of dry algae/ha/ year (Hayashi et al, 2011; Lee et al, 2016; Ra et al, 2013). Carbohydrate content of polysaccharide reaches 60.6% which consists of kappa-carrageenan and cellulose (Ra et al, 2013; Kim et al, 2015; Arad & Levy-Ontman, 2010) These carbohydrates can be broken down into monosugar through a hydrolysis process and will be fermented to produced etanol (Kim et al, 2015; Gonzalez et al, 2008; Ravanal et al, 2016; Munoz et al, 2004). Bioethanol production was developed as fuel replacement fuel with fuel grade ethanol ≥ 99.5% to compensate for the scarcity of petroleum resources It can be an alternative energy because the oxygen content was high, environmentally friendly, and its energy source was can reneweble (Kumar et al, 2013; Madigan et al, 2012)

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