Production of Bioactive Compounds by Food Associated Galactomyces geotrichum 38, as Determined by Proteome Analysis.

Anna Grygier,Joanna Pawlicka-Kaczorowska,Kamila Myszka,Kinga Stuper-Szablewska,Magdalena Rudzińska,Grażyna Chwatko,Artur Szwengiel

doi:10.3390/nu11020471

Anna Grygier, Joanna Pawlicka-Kaczorowska + Show 5 more

Open Access

https://doi.org/10.3390/nu11020471

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Abstract

Fried cottage cheese is a dairy product, popular in some parts of Poland. Proteomic analysis of a culture of the mold Galactomyces geotrichum 38 isolated from fried cottage cheese was performed using UHPLC/MS. From the proteins identified, we selected those involved in the biosynthesis of bioactive compounds and those useful in industry. In the G. geotrichum 38 culture, the production quantities of vitamin B2 (224 μg/L), ergosterol (54.63 mg/kg), and trehalose (0.91 g/L) were determined by HPLC. The identified proteins were also used to prepare a hypothetical fatty acid biosynthesis pathway, and the percentage of individual sphingolipids in the culture was determined. Sphingolipids are also bioactive compounds. During culturing of G. geotrichum 38, the percentage of three sphingolipids increased. The last step of the research was to prepare a model of fried cottage cheese. The mold G. geotrichum 38, used in the process of ripening fried cottage cheese, synthesized vitamin B2 and erogsterol, which influenced the nutritional value of the product.

Highlights

Many microorganisms are capable of producing bioactive compounds [1], and only some of these possibilities are currently known
Proteomic Analysis of Proteins Synthesized by G. geotrichum 38
The present study identified the α-subunit of the acetyl-CoA carboxyl transferase protein (ACC); this is an enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA [28] as the first stage in fatty acid biosynthesis

Summary

Introduction

Many microorganisms are capable of producing bioactive compounds [1], and only some of these possibilities are currently known. The literature indicates that G. geotrichum is capable of producing peptides that inhibit angiotensin I converting enzyme [3] and polyunsaturated fatty acids (PUFA) [4]. Proteomics is an approach that can help in understanding the ability of a microorganism to produce bioactive compounds. Such research can be carried out to identify microbial strains, to analyze proteins, and to find factors determining pathogenicity and interactions with the host. Such information about proteins can lead to knowledge of the microorganism’s ability to produce bioactive compounds [5]. There are no reports in the literature describing the proteome of G. geotrichum, but on the basis of the proteomics carried out on

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