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Abstract
Production of approximately 80–160 μmol of acetic acid was observed in 1% (w/w) methanol culture media (10 ml) of thermophilic Methanosarcina sp. from which essential nutrients such as salts of NH 4 +, PO 4 3−, K +, Ca 2+ and Mg 2+ were removed. Similar acetic acid production was found when methane gas formation was controlled to approximately 50% by means of inhibitors of methyl group incorporation into coenzyme M (CoM), such as 2-bromoethanesulfonic acid, 2-chloroethanesulfonic acid and 2-mercaptopropionic acid, with varying results for different doses. Among inhibitors of methane formation from methyl-CoM, only in the presence of chloroform some acetic acid production was observed. We studied the mechanism of acetic acid formation through tracing of marked methyl radicals in a culture medium containing 5 μmol of 2-bromoethanesulfonic acid, using 13C methanol and nuclear magnetic resonance (NMR).
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