Production of a new enzyme, nucleoside oxidase, by Pseudomonas maltophilia LB-86.

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A screening test was done to isolate microorganisms that produced nucleoside oxidase. The enzyme activity was found in a few strains of Gram-negative bacteria. One strain (LB-86) of these microorganisms showed strong ability to produce the enzyme. The morphological and physiological characteristics of strain LB-86 were studies, and this strain was found to be Pseudomonas maltophilia. From the examinations of culture conditions, suitable conditions for enzyme production were found, and strain LB-86 was aerobically cultured in shaking flasks at 26°C in a medium containing 2.5 % yeast extract, 3% glucose, 0.1% KH2P04, 0.1% KC1, and 0.05% MgS04–7H20, pH 7.2. The highest activity was obtained after 22 ~ 24 hr of cultivation.