Abstract
Biomass, the most abundant carbon source on the planet, may in the future become the primary feedstock for production of fuels and chemicals, replacing fossil feedstocks. This will, however, require development of cell factories that can convert both C6 and C5 sugars present in lignocellulosic biomass into the products of interest. We engineered Saccharomyces cerevisiae for production of 3-hydroxypropionic acid (3HP), a potential building block for acrylates, from glucose and xylose. We introduced the 3HP biosynthetic pathways via malonyl-CoA or β-alanine intermediates into a xylose-consuming yeast. Using controlled fed-batch cultivation, we obtained 7.37±0.17g 3HPL−1 in 120hours with an overall yield of 29±1%Cmol 3HPCmol−1 xylose. This study is the first demonstration of the potential of using S. cerevisiae for production of 3HP from the biomass sugar xylose.
Highlights
In order to maintain and improve our current living standard it is necessary with a transition to a sustainable fossil-free society, and this will require novel technologies for production of energy, fuels and chemicals
3-Hydroxypropionic acid (3HP) is an attractive bio-based platform chemical as it can be chemically converted into acrylic acid, acrylic esters and amides, and hereby enables sustainable production of superabsorbent polymers, plastics, paints, etc
Engineering 3HP pathways into the xylose-consuming yeast
Summary
In order to maintain and improve our current living standard it is necessary with a transition to a sustainable fossil-free society, and this will require novel technologies for production of energy, fuels and chemicals. 3-Hydroxypropionic acid (3HP) is an attractive bio-based platform chemical as it can be chemically converted into acrylic acid, acrylic esters and amides, and hereby enables sustainable production of superabsorbent polymers, plastics, paints, etc. We explored the production of 3HP from D-xylose by engineered S. cerevisiae. Reference Peter Kötter (Johann Wolfgang GoetheUniversity Frankfurt, Germany Scalcinati et al (2012)
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