Abstract
Summary In anticipation of experimental preparation of reagents for in vitro analysis, a pilot study was conducted at the department of radioimmunoassay kits production in collaboration with the National program of blood transfusion (PNTS) focused on the local production of polyclonal antisera anti-A and anti-B by immunization of living rabbits. This production of polyclonal antisera is an important alternative to the manufacture of sera-based assays of monoclonal antibodies obtained by hybridoma technology, expensive for our country and requiring specially trained personnel. The polyclonal antisera (anti-A and anti-B) produced in our laboratory respectively recognized the blood group antigens A and B and met the requirements and characteristics related to the preparation of reagents ABO blood group. However, a low reactivity of the antiserum anti-A was observed in vitro. This lack could be overcome by associating the techniques of neutral salt concentration of ammonium sulfate and column purification using G protein to improve significantly the analytical quality and macroscopic antisera produced locally. This study deserves to alleviate the problems of shortage of stock due to limited financial resources of our country and to ensure a permanent self-sufficiency in primary medical testing reagents commonly used for determination of ABO blood groups according to the method of Beth-Vincent in saline.
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