Production, Characterization and Application of Inulinase from Pseudozyma sp. CCMB 300.

Gildomar Valasques Junior,Rodrigo De Queiroz Oliveira,Danyo M Lima,Ana Paula Trovatti Uetanabaro,Rita De Cã¡Ssia L Figueiredo Ribeiro,Pedro Fernandes,Sandra A Assis,Diego Sampaio Nascimento,Aristã³Teles Gã³Es-Neto

doi:10.24297/jbt.v4i2.5003

Abstract

The use of inulinases provides an alternative way to obtain fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and application of an inulinase from Pseudozyma sp CCMB 300 isolated from Brazilian semi-arid region. The higher production of inulinase in a medium containing 0.86 g L-1 yeast extract and 14.54 g L-1 glucose using response surface methodology (RSM). This statistical method was also used to access the optimum pH (8.28) and temperature (54ÂºC) for fermentative inulinase production. The results showed that the inulinase has potential for inulin hydrolysis, where a conversion yields of roughly 87.39 % for an initial concentration of inulin of 1% (w/v).

Highlights

The enzyme inulinase (EC 3.2.1.7) target the β-2 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose [1].Inulin is a naturally occurring polyfructan in plants consisting of linear chains of β (2,1)-linked fructose residues attached to a terminal sucrose molecule
In the second and third column are the values of glucose concentration and the concentration of yeast extract, respectively
The Lineweaver-Burk plot showed that the Km and Vmax values of the enzyme for inulin were 23 mg/mL and 28.33 μmol/mL.min, respectively (Fig. 08). These results demonstrate that the inulinase from Collection of Microorganisms of Bahia (CCMB) 300 displayed affinity for inulin and are in agreement with other studies

Summary

Introduction

Inulin is a naturally occurring polyfructan in plants consisting of linear chains of β (2,1)-linked fructose residues attached to a terminal sucrose molecule. It is produced naturally in over 36,000 plants world wide, including 1200 native grasses and cereals belonging to several genera. Chicory and Jerusalem artichoke have been proven to contain inulinase that can be purified, but according to Kochhar and Kanur [3], these sources are not as productive as the microbial one, which seem to be the only source capable of producing enough enzymes for industrial applications. In the last three decades, significant efforts have been made to find the best microbial source for the extraction of inulinase [4]

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