Abstract
Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v).
Highlights
Inulinase (β-2, 1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose (Gong et al 2008)
In the second and third columns we have the values of glucose concentration and the concentration of yeast extract, respectively
The enzyme was afterwards used in the hydrolysis of inulin whose a conversion yields of roughly 72.48 % for an initial concentration of inulin of 1% (w/v)
Summary
Inulinase (β-2, 1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose (Gong et al 2008).Inulinase can be derived from many micro organisms. Conventional fructose production from starch needs at least three enzymatic steps, including α-amylase, amyloglucosidades, and glucose isomerase action, yielding only 45% of fructose solutions. An alternative to this process is the hydrolysis of inulin by inulinases. The enzymatic formation of fructose from inulin has a single enzymatic step and yields up to 95% of fructose (Figueiredo-Ribeiro et al 2007). The use of microbial inulinases (EC 3.2.1.7), which yield up to 95% of pure fructose in a single-step enzymatic reaction, is a viable alternative for the production of high-fructose syrup (Gill et al 2006)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
