Abstract
Aspergillus sp NCIM 508 produced 22 U/L of extracellular ∝-mannosidase activity in a medium containing 8 % brewer's yeast cells. The optimum period and pH range for maximum production of the enzyme were 7 days and 4.0–6.0, respectively. The optimum pH and temperature for enzyme activity were 6.0 and 50°C, respectively. The enzyme was stable for 24 h at 28°C, in the pH range 6.0–7.0. The enzyme retained 100 and 65 % of its original activity after heating for 15 min at 45 and 55°C, respectively. The Km and Vmax for p-nitrophenyl-∝-D- mannoside (PNPM) were 71μM and 7.5 × 10−2 μmoles/min/mg, respectively. The enzyme was strongly inhibited by 1 mM Hg++ and Cu++ and partially by Co.++
Published Version
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