Abstract
Mice were immunized with purified sperm cells using repeated subcutaneous and intraperitoneal injections. Spleen cells from immunized animals were fused by the polyethylene-glycol method with myeloma cells to develop antibody-producing hybridomas. Two fusions gave 195 stable hybridomas, and 91% produced antibodies. Antibody specificity was determined using an enzyme-linked immunosorbent assay on microtiter plates (microELISA) and immunofluorescence: 85% of all reactive antibodies bound to the sperm cell fraction, 66% to the particulate pollen fraction, and 34% to the soluble pollen fraction. While a number of antibodies bound to the three fractions, 46% were specific for the sperm cells, 8% for the particulate pollen fraction, and 16% for the soluble pollen fraction. Sixty-one percent of hybridomas produced IgM, and 28% produced IgG.
Published Version
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