Abstract
Thirteen hybridoma cell lines secreting monoclonal antibodies (MCAs) to watermelon mosaic virus 2 (WMV-2) were produced by fusing spleen cells of immunized mice and mouse myeloma cell line. The MCAs recognized at least three different epitopes on the particles of WMV-2. WMV-2 and zucchini yellow mosaic virus (ZYMV) shared one epitope (cryptotope) because a group of MCAs (group 3) to WMV-2 reacted only with ZYMV treated using carbonate buffer, pH 9.6 in non-precoated indirect enzyme-linked immunosorbent assay (I-ELISA) but not in precoated I-ELISA with rabbit antiserum to WMV-2. This fact provided a basis for clarification of the serological relationship between the two viruses. ELISA using MCA WMV-2 15 was found to be useful for WMV-2 detection and/or diagnosis of field samples.
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