전남지역의 호박에 발생하는 바이러스 병 발생 실태

Abstract

전라남도 호박 주산단지의 2000년 바이러스병 발생포장율은 노지재배 76.1%,억제재배 55%,반촉성재배는 발생이 없었으며, 포장별 이병주율은 반촉성재배 3.6%, 노지재배 100%이었다. 포장에서 채집된 시료에 대한 RT-PCR 검정결과 노지재배는 40점 시료 중 WMV 16점, ZYMV 10점, PRSV 2점이 각각 검출되었으며, WMV, ZYMV, PRSV의 2개 또는 3개 바이러스에 중복감염된 시료도 7점이었다. 억제재배는 21점 시료중 WMV 7점, ZYMV 6점, PRSV 6점, WMV와 PRSV의 2개 바이러스에 중복감염된 시료가 1점이었으며, CMV는 검출되지 않았다. Field disease incidences of squash virus diseases in Jeonnam province were estimated to be 76.1% and of delayed planting on August-September (retarding culture) and on February-March (semi-forcing culture) on glass house were 55.0% and 0%, respectively, in 2000. Disease incidences of individual squash plant within a field were 100% and 3.6%, respectively, in wild culture and retarding culture. Total of 61 samples suspected to be infected with viruses were collected in 2000 and tested by RT-PCR using specific oligonulceotide primer sets designed for the detection of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), Zucchini yellow mosaic virus (ZYMV), Papaya ring spot virus (PRSV), Watermelon mosaic virus (WMV), and Cucumber mosaic virus (CMV). Each specific primer set for WMV, ZYMV, and PRSV amplified expected size of DNA fragments from 16, 10, and 2 samples in wild culture, respectively. Double or triple infection were observed in 7 samples tested. In contrast, each specific primer set for WMV, ZYMV, and PRSV confirmed virus infection from 7, 6, and 6 samples, respectively, in samples collected from semi-forcing culture. Double infection of WMV and PRSV was observed in only one sample. However, no DNA fragment was amplified from RT-PCR using CGMMV, KGMMV, and CMV specific oligonucleotide primer sets indicating no CGMMV, KGMMV, or CMV infection in squash fields in Jeonnam province in 2000.