Abstract
L. Blomqvist and AM. Sjögren. Production and characterization of monoclonal antibodies against Staphylococcus aureus α-toxin. Toxicon 26, 265 – 273, 1988. — Murine monoclonal antibodies against staphylococcal α-toxin were produced using a well-characterized α-toxin fragment preparation as immunizing agent. Three monoclonal antibodies were selected for anti-α-toxin ativity in an ELISA using α-toxin as antigen. The monoclonal antibodies (MAbs) belonged to different immunoglobulin classes/subclasses and showed different abilities to neutralize the hemolytic, cell-membrane-damaging, dermonecrotizing and lethal action of α-toxin. One MAb was superior to mouse polyclonal antiserum in all test systems except for hemolysis, whereas another MAb neutralized essentially as the polyclonal serum. The third MAb did not neutralize the hemolytic or dermonecrotic effect but still inhibited the lethal and membrane-damaging effect of α-toxin. These results indicate that the three MAbs recognize different epitopes on the toxin molecule and that different biological activities might correspond to these epitopes.
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