Rat Hybridomas and Rat Monoclonal Antibodies (1990)

Abstract

IMMUNOCYTOMAS in LOUVAIN (LOU) RATS. Rat Immuoglobulins. The LOUVAIN (LOU) Rats. Rat Immunocytomas (IR). PRODUCTION of RAT-RAT HYBRIDOMAS. Fusion Cell Lines. Immunization of Rats. Fusion Procedure. Selective Growth of Hybrids. Cloning. Selection of Anti-Target Antibody-Producing Hybrids. Isotyping of Rat Monoclonal Antibodies. Adaptation to In Vivo Culture. Freezing and Thawing. PRODUCTION, PURIFICATION, FRAGMENTATION and LABELING of MONOCLONAL ANTIBODIES. In Vitro Production of Rat Monoclonal Antibodies. In Vivo Production of Rat Monoclonal Antibodies. Purification of Rat Monoclonal Antibodies from Ascitic Fluid - Serum or Culture Supernatant. Quality Control of Purified Antibodies. Fragmentation of Rat Monoclonal Antibodies. Labeling of Monoclonal Antibodies. EXAMPLES of PRODUCTION and CHARACTERIZATION of RAT-RAT HYBRIDOMAS AGAINST VARIOUS Ag. Rat Monoclonal Antibodies Against Murine Immunoglobulins. Allotype Specific Monoclonal Antibodies to Rat Kappa Light Chain. Applicatio n of Rat Hybridomas to Studies on Ocular Immunobiology. Rat Monoclonal Antibodies Against Rabbit Immunoglobulin. Preparation of Anti-Human IgG Monoclonal Antibodies Using Rat x Rat Hybridoma System. Rat Monoclonal Antibodies Against Human Immunoglobulin. Production and Characterization of Rat Monoclonal Antibodies Directed Against Human Apolipoprotein B. Studies on Rat-Rat Hybridoma Technique and its Application to Obtain Rat Monoclonal Antibodies Anti-Horse-radish Peroxidase. Rat-Rat and Rat-Mouse Hybridomas Secreting Rat Monoclonal Anti-Horse-radish Peroxidase. LOU Rat Monoclonal Antibodies Against Xenopus Vitellogenin. Production and Characterization of Rat-Rat Hybridomas Against DNP-Hapten. Rat x Rat Hybridomas Against Cellular Antigens. Rat Monoclonal Antibodies Against Human Leukocyte Differentiation Antigens. Rat Monoclonal Antibodies Specific for T Lymphocytes. ANTIBACTERIA ANTIGENS. Rat Monoclonal Antibodies Against Streptococcus mutans and Bacteroides gingivalis. A Specific Rat Monoclonal Antibody Against Yersinia enterocolitica Serogroup 0:8. Production of Rat Hybridoma Directed Against Yersinia enterocolitica. Rat Monoclonal Antibodies Against Viral Antigens. Contribution of Rat Monoclonal Antibodies to the Study of Schistosoma mansoni Infections. SPECIFIC USES and PROPERTIES of RAT MONOCLONAL ANTIBODIES. Mouse Immunoglobulin Isotyping. Humoral Immune Suppression of Mice by Monoclonal Rat Anti-Mouse |Heavy Chain Antibodies. Rat Monoclonal Antibodies Against Mouse IgE. Purification of Mouse Monoclonal Antibodies by Immunoaffinity Chromatography. Screening of Mouse Monoclonal Antibodies. Use of Rat Monoclonal Antibodies in Immunohistochemistry Investigations. Use of Rat-Rat Monoclonal Antibodies Labeled with Fluorochrome. Use of Rat Monoclonal Anti-Rat Allotype Antibodies in Immunohistoperoxidase Technique and ELISA. Use of Anti-Rat Kappa Light Chain Allotype Monoclonal Antibodies for the Quantification of Rat Monoclonal Antibodies in As citic Fluid or Serum