Production and characterization of exopolysaccharides from salinity-induced Auxenochlorella protothecoides and the analysis of anti-inflammatory activity

Abstract

The set scenario of this work was to investigate the production, physicochemical characteristics, and anti-inflammatory activities of exopolysaccharides from salinity-induced Auxenochlorella protothecoides. The results demonstrated that 10 ‰ salinity manipulation endowed preferable exopolysaccharide production by A. protothecoides. Under this salinity stress, ACPEPS1A and ACPEPS2A were purified from exopolysaccharide production by anion chromatography and molecular exclusion chromatography. ACPEPS1A exhibited a molecular weight (Mw) of 132 kDa and mainly consisted of galactose. ACPEPS2A was a heteropolysaccharide with an Mw of 170 kDa and the main monosaccharides of galactose and rhamnose with separate molar percents of 42.41 % and 35.29 %, respectively. FTIR, 1H and 13C NMR supported that monosaccharide components of ACPEPS1A and ACPEPS2A possessed both α- and β-configuration pyranose rings. Further evidence indicated that ACPEPS1A and ACPEPS2A could effectively inhibit the inflammatory response in lipopolysaccharide (LPS) induced RAW264.7 cells by quenching inflammatory factor levels such as ROS, iNOS, TNF-α, and IL-6. The potential anti-inflammatory possibilities were that the monosaccharides of ACPEPS1A and ACPEPS2A possessed higher affinity with receptors on the macrophage surface than LPS and hampered LPS-induced inflammation. The findings of this work would favor innovative applications of exopolysaccharides from microalgae in complementary medicines or functional foods.