Production of exopolysaccharides from novel marine bacteria and anticancer activity against hepatocellular carcinoma cells (HepG2)

Mohsen S Asker,Osama H El Sayed,Salma M Abdelnasser,Sahar S Mohamed,Manal S Selim,Mostafa M Abo Elsoud,Shaymaa M Yahya,Mohamed S El Awady,Manal G Mahmoud

doi:10.1186/s42269-018-0032-3

Mohsen S Asker, Osama H El Sayed + Show 7 more

Open Access

https://doi.org/10.1186/s42269-018-0032-3

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Abstract

BackgroundThe aim of the current study based on the production and characterization of exopolysaccharides (EPSs) isolated from marine sediment of the Mediterranean and Red Seas is to study its cytotoxic activity against HepG2 cells.ResultsEleven isolates have the ability to produce EPSs and also decreased the viability of HepG2 cell line in different manners. The five most promising isolates that produce high yield of EPSs and high cytotoxicity were identified by 16S RNA as Brevundimonas subvibrioides MSA1, Bacillus thuringiensis E4, Bacillus amyloliquefaciens MGA2, Pseudomonas fluorescens SGA3, and Advenella Kashmirensis NRC-7. The chemical composition of the following EPSs (M1, M3, M6, M15, M19, E2, E4, E10, S5, S7, and S11) demonstrates that they are acidic sulfated heteropolysaccharides with different relative ratios of monosugars of glucose, mannose, galactose, glucouronic acid, and mannouronic acid. The average molecular weights from 1.94 × 104 to 7.95 × 105 g/mol and the number average molecular weight from 1.51 × 104 to 7.53 × 105 g/mol. FTIR spectrum of the five EPSs indicated the presence of sulfate and carboxylic groups in different percentages.ConclusionsThe EPSs produced from marine bacteria are very promising for treating the HepG2 cells.

Highlights

The aim of the current study based on the production and characterization of exopolysaccharides (EPSs) isolated from marine sediment of the Mediterranean and Red Seas is to study its cytotoxic activity against HepG2 cells
Natural products have been the pillar of tumor chemotherapy for as far back as 30 years and are probably going to give a considerable lot of the lead structures, and these will be utilized as layouts for the development of novel compounds with upgraded organic properties (Wani et al 1971)
Screening for production of EPSs Twenty isolates of marine bacteria were isolated from Marsa-Alam, El-Ain El-Sokhna, and Sidi Bishr

Summary

Introduction

The aim of the current study based on the production and characterization of exopolysaccharides (EPSs) isolated from marine sediment of the Mediterranean and Red Seas is to study its cytotoxic activity against HepG2 cells. In spite of the fact that chemotherapy is one of the compelling medication strategies against liver cancer, chemotherapeutic operator prompts serious unfavorable impacts while murdering tumor cells (Drake and Antonarakis 2010). Natural products have been the pillar of tumor chemotherapy for as far back as 30 years and are probably going to give a considerable lot of the lead structures, and these will be utilized as layouts for the development of novel compounds with upgraded organic properties (Wani et al 1971). The marine condition is a rich wellspring of remarkable bioactive compounds, for example, exopolysaccharides (EPSs) from microorganisms with boundless chemical and functional varieties.

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