Production and application of glucose isomerase from Streptomyces enissocaesilis and amylase from Streptomyces sp. for the synthesis of high fructose corn syrup

Abstract

This study reports Streptomyces sp. strain T.S.A.KP identified as Streptomyces enissocaesilis isolated from soil inhabiting actinobacteria of Madhya Pradesh (India) as a new actinobacterial strain for production of glucose isomerase (GI) and Streptomyces sp. strain TSA. Dm1 closely related to Streptomyces sampsonii for the production of amylase. These strains were selected from an array of 120 actinobacterial isolates, which on screening originally yielded 18% GI producers and 36% amylase producers. S. enissocaesilis yielded maximum GI at pH 7 and 70 °C in 120 h. Highest amylase production from Streptomyces sp. strain TSA. Dm1 was observed at pH 7 and 37 °C in 96 h. Michaelis–Menten constant (Km) for GI and amylase was 1.54 M and 0.36 M, respectively. Vmax for GI was 11.76 U/ml and amylase 33 U/ml. These cultures were employed for HFCS production. Saccharification of corn starch was achieved up to 90.9% by Streptomyces sp. strain TSA. Dm1, and the glucose was isomerized to 40.8% by S. enissocaesilis. Presence of glucose and fructose in HFCS was also confirmed by high-performance liquid chromatography.