Abstract
Abstract Glucose isomerase (GI) converts glucose into fructose by a reversible reaction. This reaction is industrially valuable for the production of high fructose corn syrup (HFCS) which is used as a sweetener by food and beverage industries. GI being an expensive enzyme necessitates its usage in immobilized form for increasing its reusability and economic viability. Global HFCS production accounts to 10 million tons which is highest among products yielded by immobilized process. Current study evaluates different encapsulation systems for immobilization of GI produced by Streptomyces sp. T.S.A.KP which was found to be Streptomyces enissocaesilis (Accession number MN911386 ). This is the first report of extracellular GI production by a strain of Streptomyces enissocaesilis. GI was immobilized using chitosan, alginate and agar to enhance its stability and conversion efficiency. The concentration of polymeric gel with cross linking agent was optimized to get ideal pore size for maximum GI activity. Immobilization on chitosan significantly increased (P
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