Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1.

H. A. Modi,Sheetal Bhasin

doi:10.1155/2012/874152

Abstract

The combination of medium ingredients has a profound influence on the metabolic pathways running in the microorganism which regulates the production of numerous metabolites. Glucose isomerase (GI), an enzyme with huge potential in the market, can isomerise glucose into fructose. GI is used widely for the production of High-Fructose Corn Syrup (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. Streptomyces are well-known producers of numerous enzymes including glucose isomerase. An array of 75 isolates was screened for the production of glucose isomerase. The isolate Streptomyces sp. SB-P1 was found to produce maximum amount of extracellular GI. Sucrose and raffinose among pure carbon sources and corn cob and wheat husk among crude agro residues were found to yield high enzyme titers. Potassium nitrate among pure nitrogen sources and soy residues among crude sources gave maximum production. Quantitative effect of carbon, nitrogen, and inducer on GI was also determined. Plackett-Burman design was used to study the effect of different medium ingredients. Sucrose and xylose as carbon sources and peptone and soy residues as nitrogen sources proved to be beneficial for GI production.

Highlights

Glucose isomerase (GI) (EC 5.3.1.5) catalyses the conversion of glucose into fructose
The most important application of GI is the production of High-Fructose Corn Syrup (HFCS)
Ge et al [2] developed a process for continuous production of HFCS by Immobilised Glucose Isomerase (IGI)

Summary

Introduction

The most important application of GI is the production of High-Fructose Corn Syrup (HFCS). It is an equilibrium mixture of fructose and glucose. HFCS has wide applications in pharmaceutical and food industries It is added in medicated syrups, beverages, baking, canning and confectionary items as a sweetening agent [1]. Ge et al [2] developed a process for continuous production of HFCS by Immobilised Glucose Isomerase (IGI). Marshall and Kooi in 1957 [4] for the first time reported the production of glucose isomerase from Pseudomonas hydrophila. India being an agrobased country is a rich producer of agroresidues. These residues can be utilized for microbial production processes

Materials and Methods

Result and Discussions

Quantitative Effect of Best Carbon Source

Quantitative Effect of Best Nitrogen Source

Findings

Conclusions