Abstract
An effective biosurfactant-producing bacteria, isolate L5, was isolated from mangrove sediments from both east coast and west coast of Southern of Thailand. Analysis of the 16S rRNA gene sequence confirmed that isolate L5 was Agrobacterium rubi with 100% homology. The biosurfactant production was performed using a mineral salt medium (MSM) with molasses as a carbon and commercial monosodium glutamate (MSG) as nitrogen sources. Under optimized conditions, A.rubi L5 was able to grow and produce biosurfactant with the yield of 4.62 g/l at 54 h of cultivation. It could reduce the surface tension of pure water from 72.0 to 25.5 mN/m and exhibit emulsification activity toward palm oil with 65.4%. The biosurfactant found to be stable even under extreme pH, temperature and salinity conditions. The results revealed the potential use of a biosurfactant produced by A. rubi L5 to enhance mobilization sorbed motor oil from environment in comparison with those of synthetic surfactants, i.e. a nonionic surfactant Tween 80 and anionic surfactants sodium dodecyl sulfate.
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