Abstract
Production of l-glutamic acid (GA) was accomplished by using Brevibacterium flavum (DSM 20411) in 3.5 dm 3 laboratory scale bioreactors at 30°C, initial pH 7.2, penicillin ratio 10 000 U g −1 DW, agitation rate 150–500 min −1, and air flow rate 0.1–0.2 vvm. Cell and GA concentrations and concentrations of other amino acids and organic acids were measured throughout batch fermentations. By-products varied in number and concentration depending on the amount of oxygen supplied to the broth. Very low oxygen transfer rate favoured pyruvic acid and lactic acid production; while at higher oxygen transfer levels α-ketoglutaric acid and succinic acid were favoured. The 0.1 vvm and 250 min −1 conditions were optimum for maximum GA production and selectivity.
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