Abstract
Pichia fermentans ITD-00165 is a yeast with a high potential to produce isoamyl acetate, but no previous works report its growth in continuous culture. A bioreactor containing a chemically defined medium allowed continuous cultures to develop three dilution rates (0.25, 0.30 y 0.35 h-1), measuring the isoamyl acetate production. Steady-state isoamyl acetate concentration was measured using an entrapping system based on a bubbling column with n-decane. Results demonstrate that the entrapping system showed a linear correlation between the isoamyl acetate concentration into the culture medium and the absorption rate into the column. Airstream for oxygen supply was used to continuously feed isoamyl alcohol to the culture to diminish the inhibitory effect of the precursor. Finally, it was demonstrated that the yeast used the precursor to produce isoamyl acetate (0.144 mg L-1).
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