Prodigiosin of Serratia marcescens ZPG19 Alters the Gut Microbiota Composition of Kunming Mice.

Xue Li,Jing Zhang,Jie Zhang,Baolei Jia,Xiaoling Zhu,Qingshuang Chen,Xinfeng Tan

doi:10.3390/molecules26082156

Abstract

Prodigiosin is a red pigment produced by Serratia marcescens with anticancer, antimalarial, and antibacterial effects. In this study, we extracted and identified a red pigment from a culture of S. marcescens strain ZPG19 and investigated its effect on the growth performance and intestinal microbiota of Kunming mice. High-performance liquid chromatography/mass spectrometry revealed that the pigment had a mass-to-charge ratio (m/z) of 324.2160, and thus it was identified as prodigiosin. To investigate the effect of prodigiosin on the intestinal microbiota, mice (n = 5) were administered 150 μg/kg/d prodigiosin (crude extract, 95% purity) via the drinking water for 18 days. Administration of prodigiosin did not cause toxicity in mice. High-throughput sequencing analysis revealed that prodigiosin altered the cecum microbiota abundance and diversity; the relative abundance of Desulfovibrio significantly decreased, whereas Lactobacillus reuteri significantly increased. This finding indicates that oral administration of prodigiosin has a beneficial effect on the intestinal microbiota of mice. As prodigiosin is non-toxic to mouse internal organs and improves the mouse intestinal microbiota, we suggest that it is a promising candidate drug to treat intestinal inflammation.

Highlights

Academic Editor: Ricardo CalhelhaReceived: 7 March 2021Accepted: 5 April 2021Published: 9 April 2021Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Licensee MDPI, Basel, Switzerland.Prodigiosin is a red microbial pigment with a tripyrrole ring structure [1,2,3]
Phology and physiological and biochemical characteristics combined with gene phology and physiological and biochemical characteristics combined with 16S rRNA gene identification, the identified as as
Assisted acid methanol extraction to extract prodigiosin from the bacteria, and was sonic‐assisted acid methanol extraction to extract prodigiosin from the bacteria, and used to determine that the prodigiosin had a purity of 95.16%

Summary

Introduction

Prodigiosin is a red microbial pigment with a tripyrrole ring structure [1,2,3]. It is mainly produced by Serratia marcescens [4,5], Pseudomonas, Actinomycetes, and some marine bacteria [6,7,8]. Prodigiosin appears red under acidic and neutral conditions and yellow under alkaline conditions [9,10]. Prodigiosin inhibits algae growth and is used to control natural water pollution, such as that caused by red tides and blooms [17]. Prodigiosin has broad application prospects in the fields of medicine, environmental management, and cosmetics

Methods

Results

Conclusion