Abstract
To investigate the differences in intestinal microbiota between diarrhea irritable bowel syndrome mice (IBS-D) and healthy mice and to explore the effects of Jianpi mixture on intestinal microbes' changes in IBS-D mice based on 16S rDNA sequencing analysis. 48 young ICR male mice were randomly divided into four groups (n = 12): (1) control group, (2) IBS-D group fed with distilled water, (3) IBS-D group fed with lactic acid bacteria compound, and (4) IBS-D group fed with Jianpi mixture for 14 days. At the end of the treatment period, 5 mice were randomly selected from each group, and then the changes in intestinal microbiota in the mice before and after treatment were analyzed by 16S rDNA high-throughput gene sequencing. Compared with the control group, the species richness and species diversity of intestinal microbiota in feces and intestinal mucosa of IBS-D mice were decreased (P < 0.05); IBS-D mice showed changes in composition of and in ratio of the intestinal microbiota in feces and intestinal mucosa at the level of phylum, class, order, family, genus, and species. Treatment with Jianpi mixture increased the species diversity of intestinal microbiota in IBS-D mice (P < 0.05) and the abundance of beneficial bacteria (P < 0.05) and decreased the abundance of harmful bacteria (P < 0.05) at the level of phylum and genus. Compared with healthy mice, the species richness and species diversity of intestinal microbiota of IBS-D mice are decreased. The intervention with Jianpi mixture can improve its diversity and regulate the equilibrium between beneficial and harmful bacteria.
Highlights
Irritable bowel syndrome (IBS) is a functional bowel disease characterized by abdominal pain and bloating discomfort accompanied by changes in bowel habits and feces characteristics
irritable bowel syndrome (IBS-D) has high incidence and significant negative impact on the quality of life and social activities of the patients. e pathogenesis of IBS-D is not completely clear, and it is mainly caused by genetic factors, gastrointestinal dysfunction, visceral hypersensitivity, intestinal inflammation, immune dysfunction, and brain-gut axis feedback abnormalities [2]. e traditional opinion is that IBS-D is a functional bowel disease, but more and more studies have revealed that the pathogenesis of IBS-D is an immune-inflammation-related disease, which is a local, sustained, low-grade inflammatory response of the intestine caused by microorganisms
It can be seen that diarrhea in mice in compound of the lactic acid bacteria group and in Jianpi mixture group had been improved after treatments. e feces of the IBS-D group were still sparse and not formed, and the hair was gray and temperate. e hair and behavioral status of the compound of lactic acid bacteria group and Jianpi mixture group were improved compared with the IBS-D group. e feces of the compound lactic acid bacteria group began to be normal after 7 days, and the feces of the Jianpi mixture group are formed, but the water was still more
Summary
Irritable bowel syndrome (IBS) is a functional bowel disease characterized by abdominal pain and bloating discomfort accompanied by changes in bowel habits and feces characteristics. E traditional opinion is that IBS-D is a functional bowel disease, but more and more studies have revealed that the pathogenesis of IBS-D is an immune-inflammation-related disease, which is a local, sustained, low-grade inflammatory response of the intestine caused by microorganisms. It has been reported that plus Sijunzi decoction can ameliorate the main symptoms of IBSD patients, its effect is slow but excellent, and the efficiency is stable, which is worthy of clinical application [5]. E Jianpi mixture applied in the present study is based on Sijunzi decoction with modifications and is found to have a satisfied curative effect on the treatment of IBS-D patients. Is study used 16SrDNA high-throughput gene sequencing technology to observe the changes in intestinal microbiota in IBS-D mice. The effect of Jianpi mixture on the intestinal microbiota of mice was observed and analyzed
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