Abstract
The release of ribosomal subunits from isolated liver nuclei is stimulated by macromolecules in the cytosol, the concentration‐dependence curve being sigmoidal in character. These factors appear to be proteins which adsorb to DEAE‐cellulose at pH 8.0, precipitate between 20 and 60% saturation with ammonium sulfate and are partially denatured during a short incubation at 50 °C, or during prolonged storage at ‐15 °C. Pre‐incubation of the nuclei at 30 °C for 10 min prior to the addition of ATP neither resulted in release of RNA, nor affected the subsequent rate of release when the energy source was added. The degree of processing of the 45‐S ribosomal precursor RNA was also directly or indirectly dependent on the presence in the incubation medium of ATP and cytoplasmic macromolecules.
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