Abstract
The cellulase auxiliary ultrasonic-assisted extraction process of salidroside from Rhodiola cretinii was optimized by response surface methodology. The extraction content of salidroside was measured by HPLC, On the basis of single factor experiment, extraction time, quantity of cellulase, extraction temperature and ethanol concentrations were selected as independent variables, and the extraction content of salidroside was selected as response value, Box-Behnken design was adopted and optimized extraction process in this experiment, the optimum extraction conditions were confirmed. The results showed that the optimu extraction conditions were as follows: 29 min, 0.18% cellulose, 43°C, 39% ethanol concentration, the extraction content of salidroside was 4.49 mg/g. Through the verified tests, the content of salidroside was 4.39 mg/g after cellulase was added, the content of salidroside without cellulase was 0.48 mg/g. The response surface analysis can predict the experimental results well, and this experiment can provide a more reasonable and effective method for extracting salidroside from R. cretinii.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
