Process Optimization of Nuciferine with Cellulase Auxiliary Ultrasonic-Assisted Extraction from Leaves of Nelumbo Nucifera Using Response Surface Methodology

Abstract

The cellulase auxiliary ultrasonic-assisted extraction process of nuciferine from Nelumbo nucifera leaves was optimized by response surface methodology. The extraction content of nuciferine was measured by HPLC, On the basis of single factor experiment, ethanol concentration, liquid-solid ratio, and extraction times were selected as independent variables, and the extraction content of nuciferine was selected as response value, Box-Behnken design was adopted and optimized extraction process in this experiment, the optimum extraction conditions were confirmed. The results showed that the optimum extraction conditions were 10% cellulase, 87% ethanol concentration, 1:26g/mL solid-liquid ratio, 3 times and 25 minutes. The content of nuciferine was 0.343mg/g. The extraction rate of nuciferine was 0.034% after cellulase was added, the extraction rate of nuciferine without cellulase was 0.010%. The extraction process obtained was reasonable, this experiment can provide a more excellent and reliable method for extracting nuciferine from leaves of Nelumbo nucifera.