Process Optimization of Salidroside with Cellulase Auxiliary Ultrasonic-Assisted Extraction from Rhodiola Cretinii by Response Surface Methodology

Abstract

The cellulase auxiliary ultrasonic-assisted extraction process of salidroside from Rhodiola cretinii was optimized by response surface methodology. The extraction content of salidroside was measured by HPLC, On the basis of single factor experiment, extraction time, quantity of cellulase, extraction temperature and ethanol concentrations were selected as independent variables, and the extraction content of salidroside was selected as response value, Box-Behnken design was adopted and optimized extraction process in this experiment, the optimum extraction conditions were confirmed. The results showed that the optimu extraction conditions were as follows: 29 min, 0.18% cellulose, 43°C, 39% ethanol concentration, the extraction content of salidroside was 4.49 mg/g. Through the verified tests, the content of salidroside was 4.39 mg/g after cellulase was added, the content of salidroside without cellulase was 0.48 mg/g. The response surface analysis can predict the experimental results well, and this experiment can provide a more reasonable and effective method for extracting salidroside from R. cretinii.