Abstract
The determination of radioactive amino acid incorporation into proteins is important in measurements of protein degradation. Aminoacyl-tRNAs participation in label distribution may interfere during these measurements. To check this possibility, a separate procedure with ion-exchange paper DE 81 allowing the percentage of radioactivity in aminoacyl-tRNA to be determined was developed. After application of microliter amounts of Trametes versicolo myceloum extracts on DE 81 paper circles, stepwise elution with buffers of different pH's and ionic strengths was carried out. This enabled labelled proteins to be separated from labelled aminoacyl-tRNAs and unbound amino acids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
