Abstract
Abstract Introduction Streptococcus salivarius is a dominant oral species and the best suitable candidate for probiotic of the oral cavity. Since Streptococcus salivarius is able to produce bacteriocins against Streptococcus pyogenes interest has been focused on the use of it as a probiotic to avoid sore throats by Streptococcus pyogenes. Objective This study is for selecting Streptococcus salivarius strains for potential use as probiotics for the oral mucosa, that is, production of bacteriocin against Streptococcus pyogenes and the ability to bind to KB cells. Material and method Tongue material from 45 students was collected and seeded on Mitis Salivarius Agar plaques. The strains were tested by the production of bacteriocin-like substances (BLIS) against S. pyogenes, biochemically and PCR for identification of S. salivarius. The best strains were tested for adherence to KB cells. Briefly, S. salivarius strains were cultured in broth, washed and suspended at 108cells/ml. KB cells were inoculated into plaques, washed and incubated with the bacteria, for adhesion. These were washed for lysis of the KB cells and release bacteria for determination of CFU. Result The bacteriocin test showed that 133 strains presented inhibition of S. pyogenes. The samples tested for adhesion to KB cells, presented different profiles and only three strains presenting high adhesion capacity. Conclusion The selection of strains of Streptococcus salivarius with high inhibitory activity against Streptococcus pyogenes, as well as adherence to KB cells leads us to the next future step, that is, to use the best strains for in vivo colonization tests
Highlights
Streptococcus salivarius is a dominant oral species and the best suitable candidate for probiotic of the oral cavity
Some strains of Streptococcus salivarius are able to produce bacteriocins that inhibit the growth of Streptococcus pyogenes in vitro
We have reported that 98% of the children who had never developed sore throat presented Streptococcus salivarius bacteriocin-like substances (BLIS) positive strains in their tongues[5]
Summary
Streptococcus salivarius is a dominant oral species and the best suitable candidate for probiotic of the oral cavity. Objective: This study is for selecting Streptococcus salivarius strains for potential use as probiotics for the oral mucosa, that is, production of bacteriocin against Streptococcus pyogenes and the ability to bind to KB cells. We have reported that 98% of the children who had never developed sore throat presented Streptococcus salivarius BLIS positive strains in their tongues[5]. The presence of this bacteria in the mouth can prevent pharyngeal infections, especially in school-aged children[6]. The present study investigated the production of inhibitory substances by S. salivarius isolated from the tongue of healthy children and the ability of these strains to adhere to KB cells
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