Probiotic Lactobacillus spp. act Against Helicobacter pylori-induced Inflammation.

Yi-Hsing Chen,Wan-Hua Tsai,Hsin-Chih Lai,Yu-Hsin Lin,Chih-Ho Lai,Tzu-Lung Lin,Hui-Yu Wu,Wen-Ling Yeh,Yu-Wen Chen,Wen-Wei Chang,Ya-Hui Chen,Hui-Ying Hsu,Wei-Wei Chen,Chun-Ya Chen

doi:10.3390/jcm8010090

Abstract

The bacterial species, Helicobacter pylori, is associated with several gastrointestinal diseases, and poses serious health threats owing to its resistance to antibiotics. Lactobacillus spp., on the other hand, possess probiotic activities that have beneficial effects in humans. However, the mechanisms by which Lactobacillus spp. harbor favorable functions and act against H. pylori infection remain to be explored. The aim of this study was to investigate the ability of bacterial strains, Lactobacillus rhamnosus and Lactobacillus acidophilus, termed GMNL-74 and GMNL-185, respectively, to inhibit H. pylori growth and inflammation. Our results showed that GMNL-74 and GMNL-185 possess potent antimicrobial activity against multidrug resistant (MDR)-H. pylori. In addition, an in vitro cell-based model revealed that the inhibition of H. pylori adhesion and invasion of gastric epithelial cells and interleukin-8 production were significantly decreased by treatment with both the Lactobacillus strains. In vivo studies demonstrated that colonization of H. pylori and induced inflammation in the mouse stomach were also alleviated by these Lactobacillus strains. Furthermore, the abundance of beneficial gut bacteria, including Bifidobacterium spp. and Akkermansia muciniphilia, were significantly increased in H. pylori-infected mice treated with GMNL-74 and GMNL-185. These results demonstrate that Lactobacillus spp. ameliorate H. pylori-induced inflammation and supports beneficial gut specific bacteria that act against H. pylori infection.

Highlights

Helicobacter pylori is a gram-negative, microaerophilic, spiral-shaped bacterium that colonizes the stomach mucosa and causes gastrointestinal diseases [1]
(Table S1), GMNL-74 and GMNL-185 were identified as Lactobacillus rhamnosus and Lactobacillus acidophilus, respectively
We further evaluated the inhibitory activity of these Lactobacillus strains against H. pylori growth

Summary

Introduction

Helicobacter pylori is a gram-negative, microaerophilic, spiral-shaped bacterium that colonizes the stomach mucosa and causes gastrointestinal diseases [1]. Epidemiological analyses revealed that nearly one-third of the population of North Europe and North America was infected with H. pylori, whereas in Southern and Eastern Europe, South America, and Asia, more than half the population suffered from H. pylori infection [2]. This infection is associated with atrophic gastritis [3] and gastric cancer [4,5], suggesting that eradication of H. pylori can reduce the incidence of such diseases. Two major virulent factors of H. pylori are reported to induce pathogenesis: Cytotoxin associated gene A (CagA) and vacuolating cytotoxin A (VacA).

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