Abstract
The efficacy of standard therapeutic strategies for Helicobacter pylori (H. pylori) infection is decreasing over time due to the emergence of drug-resistant strains. As an alternative, the present study investigated the capacity of Lactobacilllus paracasei (L. paracasei) HP7, isolated from kimchi, to inhibit H. pylori growth. The effects of L. paracasei HP7 on H. pylori adhesion and H. pylori-induced inflammation were examined in ACS human gastric adenocarcinoma epithelial cells and a mouse model of H. pylori SS1 infection. L. paracasei HP7 reduced H. pylori adhesion to ACS cells and suppressed the inflammatory response in infected cells by downregulating interleukin-8. H. pylori colonization in the stomach of C57BL/6 mice was demonstrated by rapid urease test, and results showed significant decrease in mice post-treated with L. paracasei HP7. Additionally, L. paracasei HP7 decreased gastric inflammation and epithelial lesions in the stomach of H. pylori-infected mice. These results demonstrate that L. paracasei HP7 treatment can inhibit H. pylori growth and is thus a promising treatment for patients with gastric symptoms such as gastritis that are caused by H. pylori infection.
Highlights
Helicobacter pylori is a Gram-negative, spiral-shaped bacterium in stomach that is the major pathogen of chronic gastric inflammation [1] and stomach ulcers [2] and is related to increased risk of stomach cancer [3,4]
To determine whether L. paracasei HP7 affects the adhesion of H. pylori to AGS cells, we examined H. pylori 16S RNA gene expression in AGS cells
To determine whether L. paracasei HP7 can block H. pylori-induced IL-8 production, AGS cells were left untreated or were pre-treated with L. paracasei HP7 prior to H. pylori infection, and IL-8 production was measured by enzyme-linked immunosorbent assay (ELISA)
Summary
Helicobacter pylori is a Gram-negative, spiral-shaped bacterium in stomach that is the major pathogen of chronic gastric inflammation [1] and stomach ulcers [2] and is related to increased risk of stomach cancer [3,4]. Lactic acid bacteria (Lactobacillus spp.) have been recommended as an additive agent in the standard recommended treatment for H. pylori therapy and can improve patient compliance by decreasing antimicrobial agents-associated side effects [15,16]. Lactobacillus salivarius was reported to inhibit H. pylori colonization. Intragastic treatment of a culture supernatant of Lactobacillus acidophilus revealed to inhibit Helicobacter felis infection [18,19]. L. acidophilus culture supernatant had a partial but longterm inhibiting effect on H. pylori infection in humans [20]. We are aimed to study that the lactic acid bacterium Lactobacillus paracasei HP7 isolated from kimchi, a fermented vegetable dish widely consumed in Korea, has inhibitory effects against H. pylori in vitro and in vivo
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