Abstract
Balanced colonization of the gastrointestinal tract in the newborn is very important for the acquisition of an immune system in infancy. Lactobacillus spp. is useful in human nutrition because of its potential probiotic and functional features. In this research, 27 strains were identified representing 104 species of Lactobacillus isolated from baby feces. The probiotic and functional properties of isolates were investigated. In this study, the sample consisted of 14 children aged 3-47 weeks who were breastfed. Strains were determined phenotypically by testing arginine hydrolysis, salt tolerance, production of gas from glucose, and growth at 15 and 45 °C. Isolated strains were genotypically characterized as Lactobacillus paracasei subsp. paracasei (41), L. casei (17), L. fermentum (24), Lactobacillus spp. (11), and L. rhamnosus (11) using 16S rDNA sequence analysis. Several strains of L. fermentum, and a majority of the strains of L. rhamnosus and L. casei/L. paracasei subsp. paracasei were able to produce hydrogen sulfide. Almost all strains showed antibacterial activity against the enteric pathogens Escherichia coli O157:H7, E. coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella Enteritidis. In this research, lactobacilli isolated from babies had probiotic properties.
Highlights
The intestinal microbiota is an active ecosystem with high complexity, which is composed of more than 400 bacterial species and an average of 1014 microorganisms (Pinto et al, 2006)
The FAO of the UN and the WHO (WHO/FAO) describe ‘probiotics’ as living microorganisms that provide a health benefit to the host when administered in adequate amounts (Munoz-Quezada et al, 2013)
Cereal products, juices, processed meat, and vegetable products, nutritional supplements, and drugs have been considered as a source of probiotic (Zendeboodi et al, 2020)
Summary
The intestinal microbiota is an active ecosystem with high complexity, which is composed of more than 400 bacterial species and an average of 1014 microorganisms (Pinto et al, 2006). This microbiota plays crucial roles in preventing colonization from potential pathogenic microorganisms and developing the immune system. Cereal products, juices, processed meat, and vegetable products, nutritional supplements, and drugs have been considered as a source of probiotic (Zendeboodi et al, 2020). Different terms about probiotics have been proposed in recent years. postbiotics (healthful metabolites of probiotics), probioactives (probitic bacterial lysates that eliciting immune response), paraprobiotics (inactivated/dead cells of probiotics) psycobiotic (mental healthful probiotics) (Champagne et al, 2018; Zendeboodi et al, 2020)
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