PROBIOTIC BIFIDOBACTERIUM AND A NEW GENERATION POSTBIOTIC: ALGINIC ACID

Abstract

Abstract AIMS OF THE STUDY According to International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus (2019),Postbiotics are defined as a preparation of inanimate microorganisms and/or their components that confers a health benefit on the host. Postbiotic Alginic acid, also called algin, is a naturally occurring, edible exopolysaccharide found in brown algae. Alginic acid and its Na/Ca salt form of Alginate show the capability in water-binding, water-retention water, and immense swelling and gelation, which could act as a protective barrier via promoting biofilm formation on the bacterial cell surfaces. Alginic acid is a potent inducer of Th1 pathway.We aimed to analyze the Postbiotic alginate formed by the human intestinal beneficial bacteria, bifidobacteria,by degrading sodium alginate. METHODS In these study, which were designed in Yildiz Technical University / Istanbul, Biohybrid films were produced by using both Bifidobacterium animalis subsp.lactis BB-12 probiotic strain and Bifidobacterium infantis in combination with sodium alginate (SA), which demonstrates biocompatibility and facilitated gelation properties in aeorobic atmospheric condition were considered as bifidobacterial surfactan source. RESULTS In biohybrid biofilm study(1), based on the spectroscopic and mechanical analysis, it was found that mechanical strength increased in films produced by adding Bifidobacterium infantis in SA while this increase was relatively lower as compared to those containing Bifidobacterium animalis subsp. lactis BB-12 as crosslinking ratio increases. Besides, bacteria contained in bio-hybrid films increased the percentage of amorphous zone of SA in SA/bacteria films, which reduced the crystallinity ratio. This indicated that crystalline chains contained in the structure of SA are degraded by probiotic bifidobacteria(Fig.1) CONCLUSIONS This is the first study that Biohybrid biosurfactan that contains Bifidobacterium infantis, Bifidobacterium animalis subsp. lactis BB-12 and SA structures coated medical/nonmedical devices and sets can be a pioneering approach to reduce carbon emissions, as well as to prevent secondary infections and increase device protection. Also, It paved the way for the postbiotic use of alginic acid/Alginate by probiotic Bifidobacteria.