Abstract
Nanomaterials are used to modify and improve the pharmacodynamic and pharmacokinetic properties of drug molecules of diverse types. The plant extracts are safe, beneficial and ecological for the synthesis of the large scale of nanomaterials. Herein, we report the new ultra fast silver nanoparticles (SNPs) synthesis via green route using aqueous Spathodea campanulata leaf extracts without using any external reducing ̸ stabilizing agent and assessment of their bio-logical application. The maximum UV–Vis spectroscopic absorbance was observed at 438 nm. EDX spectra also confirmed the construction of SNPs. TEM analysis revealed that the biogenic SNPs were of 18.4 nm in size (d). Hydrodynamic size (d = ~20 nm) was deliberated by the DLS. FT-IR study indicated that OH, NH, N-O, CO and COOH functional groups participated in the construction of SNPs. The zeta potential value (−16.8 mV) confirmed the dispersion and stability of the SNPs. The SNPs showed a dose dependent bactericidal property against Escherichia coli ATCC 25922 and MIC was found to be 1.2 nM for it. The binding of SNPs with human serum albumin (HSA) and SNPs-HSA complex were investigated by UV–Vis absorbance, fluorescence, circular dichroism (CD) spectroscopy, fluorescence lifetime and zeta potential measurement. The slight conformational changes of the protein in the presence of SNPs were confirmed by absorbance, 3D fluorescence, synchronous fluorescence and CD spectroscopy. This work improves our knowledge about the structural nature of binding between the biogenic SNPs and HSA.
Published Version
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