Probing Layered Tissues by Backscattering Mueller Matrix Imaging and Tissue Optical Clearing

Abstract

Polarization imaging is a label-free and non-invasive technique that is sensitive to microstructure and suitable for probing the microstructure of living tissues. However, obtaining deep-layer information from tissues has been a challenge for optical techniques. In this work, we used tissue optical clearing (TOC) to increase optical penetration depth and characterize the layered structures of tissue samples. Different tissue phantoms were constructed to examine changes in the polarization features of the layered structure during the TOC process. We found that depolarization and anisotropy parameters were able to distinguish between single-layer and double-layer phantoms, reflecting microstructural information from each layer. We observed changes in polarization parameter images during the TOC process and, by analyzing different regions of the images, explained the sensitivity of these parameters to double-layer structures and analyzed the influence of oblique incident illumination. Finally, we conducted TOC experiments on living skin samples, leveraging the experience gained from phantom experiments to identify the double-layer structure of the skin and extract features related to layered structures. The results show that the combination of backscattering polarization imaging and tissue optical clearing provides a powerful tool for the characterization of layered samples.