Abstract
If the plasma membrane has coexistence of liquid-disordered (Ld) and liquid-ordered (Lo) phases, then proteins can be sorted between these phases. This partition of molecules in Ld and Lo phases can be examined with fluorescent probes in model lipid mixtures that have coexisting domains. Here, we measured the partition coefficient, Kp, of Bodipy-PC and tryptophan octadecyl ester (TOE) between Ld and Lo phases for three-component lipid mixtures that exhibits macroscopic phase separation, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)/ 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/ Cholesterol (Chol) and brain sphingomyelin (bSM) /DOPC/Chol, and for mixtures that exhibit nanoscopic phase separation, DSPC/ 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/Chol and bSM/POPC/Chol.
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