Abstract
Staphylococcus aureus and Salmonella are common pathogens causing foodborne diseases, posing a serious threat to food safety. Accurate detection of foodborne pathogens is particularly critical. This study developed a probe-based dual-chip digital loop-mediated isothermal amplification (cdLAMP) technique that enables the simultaneous detection of Staphylococcus aureus and Salmonella in food samples. The chip contains 20,000 wells, each with a micro-reaction unit volume of approximately 1 nL. The dual-detection is achieved through the dual-color fluorescence channel equipped on the imaging analyzer. Compared with qPCR, the cdLAMP demonstrates superior sensitivity. In the detection of pure cultures and spiked food samples, the detection limit of two foodborne pathogens is 2 CFU/mL. In the detection of field samples, it is capable of detecting single colonies of both pathogens. The dynamic detection range spans 5 logs. In conclusion, the probe-based cdLAMP technique emerges as a user-friendly tool for detecting foodborne pathogens, providing a powerful platform for food safety testing.
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