Pristionchus trametes n. sp. (Diplogastridae) isolated from the mushroom Trametes orientalis in Kyoto, Japan.

Natsumi Kanzaki,Keiko Hamaguchi

doi:10.21307/jofnem-2021-060

Abstract

A new species of Pristionchus was isolated from fruiting bodies of the wood-decaying fungus Trametes orientalis collected from Kyoto, Japan. Attempts to culture it using bacteria, yeast, and freeze-killed wax moth larvae as food or substrate failed. The eurystomatous form of the species was not found in the collected material, and the species is typologically characterized by: its ‘small’ stoma with thin, membrane-like cheilostomatal plates, a small triangular right subventral tooth, thorn-like dorsal tooth, and small left subventral denticles; a short, blunt male tail spike; and a short, conical female tail. Although the posterior probability support was not high (66%), phylogenetic analysis of both small and large ribosomal RNA gene subunits suggests that the species is closely related to P. elegans and P. bucculentus. The new species can be distinguished from those two by its diagnostic characters comprising the stomatal morphology and male and female tail characters.

Highlights

A new species of Pristionchus was isolated from fruiting bodies of the wood-decaying fungus Trametes orientalis collected from Kyoto, Japan
This study describes a species of Pristionchus recovered from fruiting bodies of the wood-decaying fungus Trametes orientalis (Yasuda) based on its typological characters and ribosomal RNA sequences, which were used as species-specific molecular barcodes
No special permit was required for the material collection at the collection site, and no endangered or protected species was involved in the present study

Summary

Introduction

A new species of Pristionchus was isolated from fruiting bodies of the wood-decaying fungus Trametes orientalis collected from Kyoto, Japan. Attempts to culture it using bacteria, yeast, and freeze-killed wax moth larvae as food or substrate failed. The species in the genus are divergent, and the genus can be used as a model system in laboratory studies and in field ecology, evolutionary biology, and population genetics (e.g., Cinkornpumin et al, 2014; Herrmann et al, 2010; Morgan et al, 2014; Renahan et al, 2021; Rödelsperger et al, 2018). This study describes a species of Pristionchus recovered from fruiting bodies (mushrooms) of the wood-decaying fungus Trametes orientalis (Yasuda) based on its typological characters and ribosomal RNA sequences, which were used as species-specific molecular barcodes

Methods

Findings

Conclusion