Abstract
Untargeted metabolomic strategy was chosen to investigate as many small metabolites as possible in a collection of 13 varieties of conventionally grown spring and winter wheat and organic wheat (Triticum aestivum). Metabolites were separated by high-performance liquid chromatography on a reversed-phase column (RP-HPLC) coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS). The procedure includes extraction of metabolites followed by chromatographic separation using the linear gradient of aqueous formic acid and acetonitrile with subsequent identification of compounds by MS/MS. Discrimination of the metabolomic patterns of different wheat varieties was achieved by principal component analysis (PCA). Results of PCA indicated clear differences in the patterns of wheat varieties. The winter wheat grown in conventional conditions and the spring wheat grown in organic conditions differed from the spring wheat grown in conventional conditions by the higher content of carbohydrates. It could be explained by osmotic stress resistance. Varieties grown under organic conditions could be well distinguished from others by the results of PCA, which points to the existence of an impact of different farming systems.
Highlights
Untargeted metabolomic strategy was chosen to investigate as many small metabolites as possible in a collection of 13 varieties of conventionally grown spring and winter wheat and organic wheat (Triticum aestivum)
Metabolites were separated by high-performance liquid chromatography on a reversed-phase column (RP–HPLC) coupled with electrospray ionization tandem mass spectrometry (ESI–MS/MS)
The winter wheat grown in conventional conditions and the spring wheat grown in organic conditions differed from the spring wheat grown in conventional conditions by the higher content of carbohydrates
Summary
Untargeted metabolomic strategy was chosen to investigate as many small metabolites as possible in a collection of 13 varieties of conventionally grown spring and winter wheat and organic wheat (Triticum aestivum). The winter wheat grown in conventional conditions and the spring wheat grown in organic conditions differed from the spring wheat grown in conventional conditions by the higher content of carbohydrates. It could be explained by osmotic stress resistance. Varieties grown under organic conditions could be well distinguished from others by the results of PCA, which points to the existence of an impact of different farming systems. Organic agriculture is focused on varieties with ecologically better-adapted traits that yield without the use of fertilizers and pesticides, and is at the same time oriented to production of good quality. Several reports deal with outcomes of organic field trials, organic plant breeding, and crop production [1,3,7]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
