Abstract
The primary structure of sarcotoxin I, a potent bactericidal protein induced in the hemolymph of larvae of Sarcophaga peregrina (flesh fly), was investigated. Sarcotoxin I was a mixture of three proteins (sarcotoxins IA, IB, and IC) with almost identical primary structures. These proteins were found to consist of 39 amino acid residues and to differ in only 2-3 amino acid residues. The amino-terminal half of the molecules was rich in charged amino acids and was hydrophilic, whereas the carboxyl-terminal half was hydrophobic. It is suggested that the carboxyl-terminal half of sarcotoxin I penetrates into the bacterial membrane and that its amino-terminal half rich in basic amino acid residues interacts with acidic phospholipids in the bacterial membrane, resulting in perturbation of the membrane and loss of viability of the bacteria.
Highlights
I, an Antibacterial Protein Induced in the Hemolymph of Sarcophaga peregrina(Flesh Fly) the membrane may be the main reason for the bactericidal action of sarcotoxin I, since the uncA mutant of E. coli, which produced ATP v i a substrate level phosphorylation, was much less sensitive to sarcotoxin I than thewild-type strain under
The membrane may be the main reason for the bactericidal action of sarcotoxin I, since the uncA mutant of E. coli, which produced ATP v i a substrate level phosphorylation, was much less sensitive to sarcotoxin I than thewild-type strain under certain environmental and ionic conditions [12]
The present paper reports the complete primary structure of sarcotoxin I
Summary
I, an Antibacterial Protein Induced in the Hemolymph of Sarcophaga peregrina(Flesh Fly) the membrane may be the main reason for the bactericidal action of sarcotoxin I, since the uncA mutant of E. coli, which produced ATP v i a substrate level phosphorylation, was much less sensitive to sarcotoxin I than thewild-type strain under. The primary structureof sarcotoxin I, a potent bactericidal protein induced in the hemolymph of larvae a mixture of three very similar proteins each with 39 amino acid residues. In Sarcophagaperegrina, humoral antibacterial proteins are Determination of the Amino Acid Sequence of Sarcotoxin I-Each induced when the bodywall is injured with a hypodermic component of sarcotoxin I eluted from HPLC was lyophilized and needle [9].
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