Abstract
Abstract Liquid biphasic flotation (LBF) offers advanced downstream processing of bioproducts by floating of gas bubbles within the biphasic system to enhance the separation efficiency of target bioproducts. In this study, the feasibility of alcohol/salt LBF for primary capture of extracellular Bacillus subtilis xylanase from crude feedstock was investigated. The effects of types of phase-forming components; phase composition; concentration of crude feedstock load; and flotation time on the recovery performance of xylanase in LBF were evaluated. The recovery of xylanase was achieved in LBF comprised of 28 % (w/w) 2-propanol, 22 % (w/w) ammonium sulfate and 10 % (w/w) B. subtilis crude feedstock load. A maximum partition coefficient (Ke) of 13.29 ± 0.14, selectivity (S) of 24.71 ± 0.96 and recovery yield (YT) of 90.03 % ± 0.10 were recorded in the optimum 2-propanol/sulfate LBF with flotation time of 20 min. SDS-PAGE analysis revealed that B. subtilis xylanase was successfully recovered in the 2-propanol-rich top phase with high purity. The findings demonstrated that LBF is a practicable technology for the large-scale production of microbial xylanase in a one-step operation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
