Abstract
This study investigated the use of liquid biphasic flotation (LBF) which composed of alcohol and salt for betacyanins extraction from peel and flesh of red-purple pitaya (Hylocereus polyrhizus). Betacyanins have received a great deal of research attention owing to their promising antioxidant activity. The operating parameters of LBF, including types/concentrations/volumes of alcohol and salt, addition of sodium chloride, flotation time and types/concentrations of crude extract, were adopted for the betacyanins extraction. Overall, the highest values of betacyanins concentration in alcohol-rich top phase (Ct), separation efficiency (E) and partition coefficient (K) of betacyanins that reveal a maximum betacyanins extraction from the peel (95.989%, 88.361% and 24.168, respectively) and flesh (95.488%, 94.886% and 21.195, respectively) were successfully achieved with LBF. In addition, both the peel and flesh extracts of red-purple pitaya provided different hues and intensities of red color, and their antioxidant properties were well retained.
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