Abstract
Summary Somatic embryoids have been induced directly from superficial cells of the lower hypocotyl of immature sexual embryos of Brassica campestris . The response was specific to a limited period of embryonic development, but occurred on 10 of a total of 18 media tested, involving three different basal media, presence or absence of yeast extract and three hormone regimes. For one medium (B5 plus 0.05 mg · 1 −1 BAP) a spontaneous secondary cycle of direct somatic embryogenesis occurred, giving a final embryo multiplication factor of better than 150. Embryoids could be readily rooted and transferred to soil. Conditions leading to direct hypocotyl embryogenesis are discussed. The most important factor appears to be internal pre-embryogenic determined state of initiating cells. The system offers a means for minimising pollinations in the maintenance of self-incompatible inbred lines of B. campestris . Key words
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