Primary and Lateral Root Development of Dark‐ and Light‐Grown Cotton Seedlings under Salinity Stress*

Abstract

AbstractPrimary root growth dynamics and lateral root development of dark‐ and light grown cotton seedlings (Gossypium hirsutum L., cv. Acala SJ‐2) were studied under control and salinity stress conditions. The seedlings were grown by two methods: A) in paper‐lined, vermiculite‐filled beakers with the plants growing between the paper and the glass wall (Gladish and Rost, 1993), and B) in hydroponics after germination and initial growth in germination paper rolls saturated with the treatment solutions (Kent and Läuchli, 1985). After germination, daily primary root elongation rate gradually incrased to a maximum, then gradually declined to close to zero for dark‐grown seedlings, or to sustained rates of about 10 mm per day for light‐grown control plants. Salinity stress delayed primary root growth and reduced peak elongation rates, without changing the general primary root growth pattern. These results suggest that salinity changed the time‐scale, but did not modify the normal developmental sequence. Lateral root growth was more inhibited by salinity than primary root growth. In addition, elongation of lateral roots was more inhibited by salinity than their initiation and emergence. Light exposure of the shoot favored both sustained primary root growth from 7 days after planting, and lateral root emergence and growth. Salinity effects were more severe on seedlings germinated and grown in hydroponics (method B) than on vermiculite‐grown plants (method A). These results emphasize the importance of growing conditions for the NaCl‐induced effects on cotton root development. In addition, the differential effects of salinity on primary and lateral roots became evident, pointing to diverse control mechanisms for the development of these root types.