Preventive Effects of Fermented Brown Rice and Rice Bran on Spontaneous Lymphomagenesis in AKR/NSlc Female Mice

Toshiya Kuno,Satoru Takahashi,Hideki Mori,Shugo Suzuki,Hiroyuki Kato,Takuji Tanaka,Aya Naiki-Ito

doi:10.31557/apjcp.2018.19.11.3217

Abstract

Fermented brown rice and rice bran with Aspergillus oryzae (FBRA) is known to possess potentials to prevent chemical carcinogenesis in multiple organs of rodents. In the present study, possible chemopreventive effect of FBRA against spontaneous occurrence of lymphomas was examined using female AKR/NSlc mice. Four-week-old female AKR/NSlc mice were divided into three groups, and fed diets containing FBRA for 26 weeks at a dose level 0% (Group 1), 5% (Group 2) or 10% (Group 3). At the termination of experiment, the incidence of thymic malignant lymphoma of Group 3 was significantly lower than of Group 1 (p < 0.05). The average number of apoptotic cells of the thymic lymphoma of Group 3 was significantly larger than that of Group 1 (p < 0.05). In addition, the incidences of malignant lymphoma arising from body surface and abdominal lymph nodes, and the frequencies of lymphoma cell invasion to liver, kidney, spleen, and ovary of Group 3 were relatively lower than those of Group 1. These results indicate that FBRA inhibits spontaneous development of the lymphoma in female AKR/NSc mice and the inhibition of lymphomagenesis may relate to the induction of apoptosis by exposure of FBRA, suggesting that FBRA could be a protective agent against development of human lymphoma.

Highlights

The number of patients with lymphoma is reportedly increasing worldwide and approximately 300,000 new cases of lymphoma develop per year (Medeiros et al, 2017)
These results indicate that FBRA inhibits spontaneous development of the lymphoma in female AKR/NSc mice and the inhibition of lymphomagenesis may relate to the induction of apoptosis by exposure of FBRA, suggesting that FBRA could be a protective agent against development of human lymphoma
Recent epidemiological analysis indicates a clear association with socioeconomic status regarding the incidence of lymphomas; they are more common in developed nations than developing countries

Summary

Introduction

The number of patients with lymphoma is reportedly increasing worldwide and approximately 300,000 new cases of lymphoma develop per year (Medeiros et al, 2017). It is known that the incidence of lymphoma varies according to the specific type and the geographic location in the world, and the variation is likely attributable to both genetic and socioeconomic factors (Medeiros et al, 2017). In Japan, the center for cancer control and information services of the National Cancer Center reported malignant lymphoma incidences of 22.3/100,000 and 18.3/100,000 in men and women, respectively, in 2013 (Miyoshi and Ohshima, 2018). Brown rice is a staple dietary constituent in Asia, whereas rice consumed in the Western countries is generally white rice obtained from brown rice by removal of the bran. Fermented brown rice and rice bran with Aspergillus (A) oryzae (FBRA) is a food manufactured by fermenting a mixture of brown rice and rice bran with A. oryzae to improve its digestibility (Henderson et al, 2012).

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