Abstract
Aims: Determination of D variants is important for selection of blood products and to prevent anti-D-related hemolytic disease of the fetus and newborn. The prevalence of weak D phenotypes varies significantly among different ethnic populations. In Morocco, D variants have not been fully characterized. The purpose of this study was to determine prevalence of weak D among D negative and C/E+ blood donors and distribution of Rhesus blood group antigens using serologic methods. Methods: A total of 15,865 blood samples from Moroccan blood donors were tested for D, C, c, E, e antigens by routine serologic methods. Among blood donors serologically D negative C+ and/or E+, 63 samples were tested for weak D by indirect antiglobuline test, enzymatic treated cells test and adsorption elution technique. Results: Among 63 samples tested, 10 were positive by serologic methods (15.87%). Six samples identified as weak D by enzymatic test and four samples identified as Del by adsorption elution. Rhesus blood group antigens are distributed as DCcee: 38.85% and dccee :8.77%. Conclusion: The prevalence of D variants must be considered in transfusional and obstetrical strategy for RhD typing to ensure blood transfusion safety by optimizing the management of D-negative RBC units and Rh immune globulins.
Highlights
The Rh blood group system is one of the most polymorphic systems in human
The RHD gene polymorphism leads to phenotypic polymorphism D variants including weak D, Del and partial D
Among blood donors phenotyped D negative, RhC and/or RhE positive, 63 samples randomly selected were tested by indirect antiglobuline test and papain test using the manufacturer’s recommendations
Summary
The Rh blood group system is one of the most polymorphic systems in human. It is involved in the incompatible RBC transfusions conflicts and in the hemolytic disease of the newborn due to the maternalfetal blood group incompatibility [1].By year 2015, 58 Rh antigens have been identified. The Rh blood group system is one of the most polymorphic systems in human. It is involved in the incompatible RBC transfusions conflicts and in the hemolytic disease of the newborn due to the maternalfetal blood group incompatibility [1]. By year 2015, 58 Rh antigens have been identified. The most common and immunogenic are D, C, E, c, International Journal of Blood transfusion and Immunohematology, Vol 6; 2016 Partial D phenotype corresponds to qualitative changes in D antigen consistent with the absence of some epitopes [7]
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