Prevalence of virulence factors genes of Escherichia coli isolated from pregnant and non-pregnant women with urinary tract infection in Diyala Iraq

Abstract

Uropathogenic Escherichia coli (UPEC) is an essential cause factor of complicated and uncomplicated urinary tract infections. It is in charge of approximately 80% of community gained and 50% of nosocomial infection. The current study included collection of one hundred and eighty samples among different cases (pregnant and non-pregnant women) by urinary tract infection in Al – Batoul Teaching Hospital in Baquba, during the period from November 2017to February 2018. All isolates were identification as E. coli by using Gram – staining. EMB agar and conformation in VITEK2 compact system. It was found 96 E. coli isolates; it was carried out to 38 E. coli isolates. 18 isolates from pregnant and 20 isolates from non – pregnant women. In this study , E.coli isolates were resistance against different type antibiotics , it was pipracillin 84.38% ; tetracycline 70.83% ; Rifampicin 63.54% ; Levofloxacine 59.37% ; ciprofloxacin 54.17% ; tobramycin 47.9% ; Augmentin 39.58% ; gentamicin 35.49% ; chloramphenicol 30.21% ; Imipenem 26.04% .The molecular detection of virulence factors genes shows the prevalence of genes fimH , papC , hlyA , sfaS , iutA , ibeA were 100% , 94.44% , 55.5% , 72.2% , 66.66% , 22.22% respectively in pregnant case according to the mentioned tract infection. The prevalence of gene fimH , papC , hlyA , sfaS , iutA , ibeA were 85% , 60% , 25% , 45% , 45% , 5% respectively in non-pregnant women with urinary tract infection . All the studied strains showed 16 of virulence gene patterns, among isolates from pregnant 12 patterns, on the other hand the isolates from non-pregnant showed diversity of genes patterns.